The Deposition of a Lectin from
| Autor: | Keicyanne Fernanda Lessa, Dos Anjos, Cynarha Daysy Cardoso, da Silva, Mary Angela Aranda, de Souza, Alessandra Batista, de Mattos, Luana Cassandra Breitenbach Barroso, Coelho, Giovanna, Machado, Janaina Viana, de Melo, Regina Celia Bressan Queiroz, de Figueiredo |
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| Rok vydání: | 2021 |
| Předmět: |
Titanium
Nanotubes Osteoblasts Cell Survival Surface Properties technology industry and agriculture Oreochromis niloticus lectin osseointegration Cichlids Article Cell Line biocompatibility Osteogenesis Dielectric Spectroscopy Lectins Alloys Cell Adhesion Microscopy Electron Scanning TiO2 nanotubes Animals mannose-binding lectin Cell Proliferation |
| Zdroj: | Biomolecules |
| ISSN: | 2218-273X |
| Popis: | Titanium and its alloys are used as biomaterials for medical and dental applications, due to their mechanical and physical properties. Surface modifications of titanium with bioactive molecules can increase the osseointegration by improving the interface between the bone and implant. In this work, titanium dioxide nanotubes (TiO2NTs) were functionalized with a lectin from the plasma of the fish Oreochromis niloticus aiming to favor the adhesion and proliferation of osteoblast-like cells, improving its biocompatibility. The TiO2NTs were obtained by anodization of titanium and annealed at 400 °C for 3 h. The resulting TiO2NTs were characterized by high-resolution scanning electron microscopy. The successful incorporation of OniL on the surface of TiO2NTs, by spin coating, was demonstrated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIE), and attenuated total reflection-Fourier transform infrared spectrum (ATR-FTIR). Our results showed that TiO2NTs were successfully synthesized in a regular and well-distributed way. The modification of TiO2NTs with OniL favored adhesion, proliferation, and the osteogenic activity of osteoblast-like cells, suggesting its use to improve the quality and biocompatibility of titanium-based biomaterials. |
| Databáze: | OpenAIRE |
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