| Autor: |
Arnatchai, Maiuthed, Tatchakorn, Pinkhien, Supakarn, Chamni, Khanit, Suwanborirux, Naoki, Saito, Nareerat, Petpiroon, Pithi, Chanvorachote |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Anticancer research. 37(11) |
| ISSN: |
1791-7530 |
| Popis: |
A newly-synthesized derivative of renieramycin M (RM), an anticancer lead compound isolated from the blue sponge Xestospongia sp., hydroquinone 5-O-cinnamoyl ester (CIN-RM), was investigated here for its activity against non-small cell lung cancer cells.Cytotoxicity effects of CIN-RM and RM on H292 lung cancer cells were determined by the MTT assay. We also investigated the mechanism of CIN-RM-mediated apoptosis and mechanism of action of this compound by western blotting.CIN-RM showed more potent cytotoxicity than its parental compound (RM) against H292 lung cancer cells. At concentrations of 15-60 μM, CIN-RM significantly induced apoptosis by increasing expression of apoptosis-inducing factor (AIF) and activation of caspase-3 and -9. For up-stream mechanism, CIN-RM mediated apoptosis through a p53-dependent mechanism, that consequently down-regulated anti-apoptotic B-cell lymphoma 2 (BCL2), while increasing the level of pro-apoptotic BCL2-associated X (BAX). In addition, phosphorylation of pro-survival protein AKT was found to be dramatically reduced.This study revealed the potential of CIN-RM for apoptosis induction and in the development of a novel anticancer agent. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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