| Autor: |
R G, Suskind, S, Michelson-Fiske, F, Haguenau, G F, Rabotti |
| Rok vydání: |
1975 |
| Předmět: |
|
| Zdroj: |
Journal of the National Cancer Institute. 54(2) |
| ISSN: |
0027-8874 |
| Popis: |
Functional and morphologic differences between the sensitivity of nucleoli of Rous sarcoma virus-transformed cells and that of newly infected cells to the action of actinomycin D (AD) have been demonstrated by quantitative light and electron microscope autoradiography and utilized to investigate the function of the nucleolus in the early stages of infection. After a pulse exposure to low doses of AD, increased RNA synthesis is induced within 80 minutes in the fibrillar portion of the nucleolus by infection. A concomitant increase in the retention of tritiated AD in the nucleolus and a quantitative redistribution of intranuclear and cytoplasmic DNA label are interpreted as evidence for a virus-induced amplification of the binding sites of AD in nucleolar chromatin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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