| Autor: |
Iu V, Rodionov, T V, Avilova, E V, Zakharova, L S, Platonenkova, A M, Egorov, I V, Berezin |
| Rok vydání: |
1977 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 42(10) |
| ISSN: |
0320-9725 |
| Popis: |
NAD-Dependent formate dehydrogenase (FDH) has been isolated from methylotrophyc strain Bacterium sp 1 by (NH4)2SO4 fractionation of cell extract, ion-exchange chromatography and preparative isotachophoresis. Preparation of FDH is homogeneous in analytical polyacrylamide gel electrophoresis and under ultracentrifugation. Sedimentation coefficient of FDH is 4.9S. Mikhaelis constants are 1.1-10(-4) M for NAD and 1.5-10(-2) M for formate. In the absence of sulfhydril compounds FDH is unstable, but it is stable in the presence of mercaptoethanol or ditiotreitol. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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