A unique basal pattern of p53 expression in ulcerative colitis is associated with mutation in the p53 gene
| Autor: | A E, Noffsinger, J M, Belli, M A, Miller, C M, Fenoglio-Preiser |
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| Rok vydání: | 2001 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Male DNA Mutational Analysis Loss of Heterozygosity Nuclear Proteins Proto-Oncogene Proteins c-mdm2 DNA Middle Aged Immunohistochemistry Cyclins Proto-Oncogene Proteins Mutation Humans Point Mutation Colitis Ulcerative Tumor Suppressor Protein p53 Polymorphism Single-Stranded Conformational |
| Zdroj: | Histopathology. 39(5) |
| ISSN: | 0309-0167 |
| Popis: | The p53 protein is implicated in the control of cell proliferation, differentiation, and death. As part of a study characterizing p53 alterations in colonic mucosa of patients with ulcerative colitis, we identified a unique pattern of basal p53 immunoreactivity.Tissue samples (n=180) from 42 ulcerative colitis patients were evaluated for p53 alterations by immunohistochemistry, loss of heterozygosity analysis, polymerase chain reaction-single-strand conformation polymorphism and direct sequencing. In addition, the expression of the p53- associated proteins p21waf1/cip1 and MDM2 was evaluated immunohistochemically. Three basic patterns of p53 immunoreactivity were observed: (i) isolated immunoreactive cells in the crypt bases; (ii) strongly positive cells confined to the basal half of the glands; and (iii) diffusely staining cells. The basal staining pattern was observed in both non-neoplastic tissues and in some areas of dysplasia, and was associated with normal expression of p21waf1/cip1 in all cases, and with p53 mutation in seven of 11 cases.The basal pattern of p53 expression is associated with mutation in the p53 gene, and appears to be an early change in a subgroup of ulcerative colitis patients. The significance of this pattern of immunoreactivity and the mechanism by which it develops are discussed. |
| Databáze: | OpenAIRE |
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