Enzyme immobilization by covalent bonding

Autor:	M D, Trevan
Rok vydání:	2011
Zdroj:	Methods in molecular biology (Clifton, N.J.). 3
ISSN:	1064-3745
Popis:	Enzyme immobilization by covalent bonding to an insoluble polymer has the supposed advantage of irreversible binding of the enzyme to the support matrix. Many of the bonds commonly employed are not 100% stable in use, however. Unless some care is taken to ensure that the enzyme can withstand the reaction conditions, substantial loss of enzyme activity will occur. Even under favorable conditions, 60% loss of activity is not uncommon.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::88f17d47585eb2f8349b2d614489ab1e https://pubmed.ncbi.nlm.nih.gov/21400186 Zobrazit plný text záznamu