Quantitative first-pass perfusion MRI of the mouse myocardium
| Autor: | Bastiaan J, van Nierop, Bram F, Coolen, Wouter J R, Dijk, Arjan D, Hendriks, Larry, de Graaf, Klaas, Nicolay, Gustav J, Strijkers |
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| Rok vydání: | 2012 |
| Předmět: |
Gadolinium DTPA
Models Cardiovascular Contrast Media Magnetic Resonance Imaging Cine Reproducibility of Results Heart Image Enhancement Sensitivity and Specificity Mice Inbred C57BL Mice Coronary Circulation Image Interpretation Computer-Assisted Animals Computer Simulation Magnetic Resonance Angiography |
| Zdroj: | Magnetic resonance in medicine. 69(6) |
| ISSN: | 1522-2594 |
| Popis: | In this article, we present a first-pass perfusion imaging protocol to determine quantitative regional perfusion values (in mL min(-1) g(-1)) of the mouse myocardium. Perfusion was quantified using a Fermi-constrained deconvolution of the myocardial tissue response with the arterial input function. A dual-bolus approach was implemented. Experimental evidence is presented for the linearity of signal intensity in the left-ventricular lumen during the prebolus (r=0.99, P0.001) and in the myocardium during the full-bolus injection (r=0.99, P0.01) as function of Gd(DTPA)2- injection concentration used. The prebolus was used to reconstruct a nonsaturated arterial input function. Regional perfusion values proved repeatable in a cohort of nine healthy C57BL/6 mice. The perfusion values over two measurements with a 1-week interval were 7.3±0.9 and 7.2±0.6 mL min(-1) g(-1), respectively. No effects of time (P0.05) and myocardial region (P0.05) were observed. The between-session coefficient of variation was only 6%, whereas the inter-animal coefficient of variation was 11 and 8% for the separate experiments. We expect that the first-pass perfusion method here presented will be useful in preclinical studies of myocardial perfusion deficits and valuable to assess the impact of pro-angiogenic therapy after myocardial infarction. |
| Databáze: | OpenAIRE |
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