[Optimization of screening methodologies for the detection of Streptococcus agalactiae in pregnant women]
| Autor: | Silvia E, Montibello, Liliana I, Guelfand, Mónica G, Machaín, Natalia A, Carrión, María D, Ferreira, Juan C, Pidone, María E, Ceregido, Sara C, Kaufman, Rolando N, Soloaga |
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| Rok vydání: | 2010 |
| Předmět: |
Bacteriological Techniques
Pregnancy Trimester Third Argentina Rectum Temperature Sensitivity and Specificity Culture Media Streptococcus agalactiae Chromogenic Compounds Predictive Value of Tests Pregnancy Streptococcal Infections Carrier State Vagina Prevalence Humans Mass Screening Female Prospective Studies Reagent Kits Diagnostic Pregnancy Complications Infectious |
| Zdroj: | Revista Argentina de microbiologia. 43(1) |
| ISSN: | 0325-7541 |
| Popis: | Streptococcus agalactiae is a significant worldwide cause of morbidity and mortality in pregnant women and their newborn infants. The objective of this work was to determine the usefulness of bioMerieux chromogenic medium chromID Strepto B (CR) for detecting S. agalactiae in pregnant women from the selective Todd-Hewitt broth (sel-THB ) against the methods proposed by the CDC . A total of 1924 swabs were analyzed, 962 from vaginal introitus and 962 rectal, belonging to 962 women in weeks 35-37 of pregnancy. The swabs were directly seeded in CR. Both swabs were later placed in sel-THB with 15 µg/ml supplement of nalidixic acid and 10 µg/ml colistin. After 24 h of incubation, subcultures in CR medium and agar containing 5% sheep blood (SBA) were performed. The prevalence found was 17.4%. Sensitivity, specificity, positive and negative predictive values of sel-THB subcultures with CR supplement and 48 h incubation were: 98.8, 100, 100 and 99.7%, respectively. The corresponding values of direct harvest of the sample were 57.8, 100, 100, and 90%, respectively. Sensitivity of sel-THB in SBA was 85%. Sel-THB subculture performance in CR was outstanding in comparison with the method proposed by the CDC. |
| Databáze: | OpenAIRE |
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