Effects of glycyrrhizin on glucocorticoid signaling pathway in hepatocytes

Autor:	Takaharu, Yoh, Toshiaki, Nakashima, Yoshio, Sumida, Yuko, Kakisaka, Yoshiki, Nakajima, Hiroki, Ishikawa, Yoshikuni, Sakamoto, Takeshi, Okanoue, Hironori, Mitsuyoshi
Rok vydání:	2002
Předmět:	Male Receptors Glucocorticoid Hepatocytes Animals Gene Expression HSP90 Heat-Shock Proteins Rats Wistar Glycyrrhizic Acid Dexamethasone Rats Signal Transduction Tyrosine Transaminase
Zdroj:	Digestive diseases and sciences. 47(8)
ISSN:	0163-2116
Popis:	The cytoprotective effects of glycyrrhizin (GL) are similar to glucocorticoids. We investigated the effects of GL on the glucocorticoid receptor (GR) and on the enzyme activity of tyrosine aminotransferase (TAT), an hepatocyte-specific marker of glucocorticoid action, in rat hepatocytes. Pretreatment with GL significantly decreased the affinity of GRs for dexamethasone (DEX) and increased the period of time required for TAT activity to reach a peak after the addition of DEX. GL did not affect the amount of GR, but significantly decreased the amount of heat-shock protein 90 (HSP90) and HSP90-associated GR. Alternatively, TAT activity and TAT mRNA levels increased significantly after the addition of GL to hepatocytes pretreated with DEX. In conclusion, GL reduces the affinity of GRs for ligands through the decreased HSP90 expression, but significantly enhances the glucocorticoid-induced TAT-gene expression at the transcriptional level in rat hepatocytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8859a0a7679bc646a3f5d61f4a5c5a58 https://pubmed.ncbi.nlm.nih.gov/12184529 Zobrazit plný text záznamu Full text from SpringerLink