Active RhoA Exerts an Inhibitory Effect on the Homeostasis and Angiogenic Capacity of Human Endothelial Cells

Autor:	Michael, Hauke, Robert, Eckenstaler, Anne, Ripperger, Anna, Ender, Heike, Braun, Ralf A, Benndorf
Rok vydání:	2022
Předmět:	Vascular Endothelial Growth Factor A Mice rho-Associated Kinases Neovascularization Pathologic Cell Movement Human Umbilical Vein Endothelial Cells Animals Homeostasis Humans Lim Kinases rhoA GTP-Binding Protein Signal Transduction
Zdroj:	Journal of the American Heart Association. 11(12)
ISSN:	2047-9980
Popis:	Background The small GTPase RhoA (Ras homolog gene family, member A) regulates a variety of cellular processes, including cell motility, proliferation, survival, and permeability. In addition, there are reports indicating that RhoA-ROCK (rho associated coiled-coil containing protein kinase) activation is essential for VEGF (vascular endothelial growth factor)-mediated angiogenesis, whereas other work suggests VEGF-antagonistic effects of the RhoA-ROCK axis. Methods and Results To elucidate this issue, we examined human umbilical vein endothelial cells and human coronary artery endothelial cells after stable overexpression (lentiviral transduction) of constitutively active (G14V/Q63L), dominant-negative (T19N), or wild-type RhoA using a series of in vitro angiogenesis assays (proliferation, migration, tube formation, angiogenic sprouting, endothelial cell viability) and a human umbilical vein endothelial cells xenograft assay in immune-incompetent NOD
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::884ebe773aac295874121ae2eb084036 https://pubmed.ncbi.nlm.nih.gov/35699166 Zobrazit plný text záznamu Plný text