[The role of sulfhydryl groups in the functioning of DNA-dependent RNA-polymerase]

Autor:	O Iu, Chertov, O A, Seliuchenko, V M, Lipkin
Rok vydání:	1985
Předmět:	Chemistry RNA Bacterial Chemical Phenomena Escherichia coli Electrophoresis Polyacrylamide Gel Cysteine DNA-Directed RNA Polymerases Sulfhydryl Compounds Templates Genetic Oxidation-Reduction Protein Binding
Zdroj:	Bioorganicheskaia khimiia. 11(4)
ISSN:	0132-3423
Popis:	Sulfhydryl groups of Escherichia coli DNA-dependent RNA polymerase were chemically modified with alkylating and mercuric-containing compounds. Iodoacetic acid and iodoacetamide were shown not to affect the enzymatic activity, whereas N-ethylmaleimide and mercuric-containing compounds completely inhibit the RNA synthesis. RNA polymerase modified with mercuric ions looses the ability of binding with promoter--containing DNA fragments. Moreover, mercuric ions inhibit the RNA elongation stage. Suggestion is made the Cys residues of RNA polymerase play a key role in double-stranded DNA unwinding. It is shown that SH-groups of beta- and beta'-subunits participate in the binding with double-stranded fragments of DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::87f63a67266e9264571495c86de05731 https://pubmed.ncbi.nlm.nih.gov/2408626 Zobrazit plný text záznamu