A comparison of methods for measuring the antibody response in mice and cattle following vaccination against foot and mouth disease

Autor:	M J, Dus Santos, A, Wigdorovitz, E, Maradei, O, Periolo, E, Smitsaart, M V, Borca, A M, Sadir
Rok vydání:	2000
Předmět:	Rodent Diseases Mice Neutralization Tests Foot-and-Mouth Disease Vaccination Animals Cattle Diseases Cattle Enzyme-Linked Immunosorbent Assay Antibodies Viral
Zdroj:	Veterinary research communications. 24(4)
ISSN:	0165-7380
Popis:	We present a comparison of methods for evaluating the potency of foot and mouth disease vaccine in the laboratory. The anti-FMDV antibodies (Ab) in vaccinated mice were tested by liquid phase (lp) ELISA, solid phase (sp) ELISA and virus neutralization (VN), and were compared with the Ab titres detected by lpELISA, which is the official test in Argentina for testing the potency of FMD vaccines and protection against a virulent challenge in cattle. The results demonstrated that it is possible to relate the Ab levels induced in vaccinated mice with both the Ab and protective responses elicited in cattle. Furthermore, it was found that the anti-FMDV Ab titres in mice detected by lpELISA 14 days after vaccination should be an accurate parameter for predicting the results of the challenge test in cattle. Thus, this test in mice appears to be an inexpensive and rapid alternative for testing FMD vaccines in cattle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::87dfefc0b19c49b72c47d02af64ac667 https://pubmed.ncbi.nlm.nih.gov/10836271 Zobrazit plný text záznamu Full text from SpringerLink