| Autor: |
Chen-chun, Ren, Wen-jing, Wang, Guo-chuan, Liu, Hai-xia, Zhang, Yue-fan, Xu, Zhi-ming, Tang, Li-ling, Yang, Pei-jia, Chen, Bin, Li |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics. 21(4) |
| ISSN: |
1003-9406 |
| Popis: |
To isolate fetal DNA from maternal plasma and examine its fetal origin.Fetal DNA in maternal plasma was isolated from 150 samples in the first trimester and mid-trimester of pregnancy, respectively. Real-time fluorescence quantitative polymerase chain reaction PCR (FQ-PCR) was used to determine sex-determining region Y (SRY) gene on Y chromosome.Eighty-two women in the first trimester and 90 women in the mid-trimester carried male fetuses,70 and 90 samples of them were positive, respectively. The mean concentrations were (58.82+/-20.90) copies/ml and (152.08+/-62.61) copies/ml. The results of FQ-PCR were negative in the women who carried female fetuses.The results show that fetal SRY gene can be found at a time as early as 42 days of gestation in maternal plasma by the use of FQ-PCR. The number of fetal DNA increases with gestational age. The real-time FQ-PCR is of great value in the non-invasive prenatal diagnosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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