| Autor: |
Munyaradzi, Musvosvi, Huang, Huang, Chunlin, Wang, Qiong, Xia, Virginie, Rozot, Akshaya, Krishnan, Peter, Acs, Abhilasha, Cheruku, Gerlinde, Obermoser, Alasdair, Leslie, Samuel M, Behar, Willem A, Hanekom, Nicole, Bilek, Michelle, Fisher, Stefan H E, Kaufmann, Gerhard, Walzl, Mark, Hatherill, Mark M, Davis, Thomas J, Scriba, Tran, Van |
| Rok vydání: |
2021 |
| Zdroj: |
Nature medicine. |
| ISSN: |
1546-170X |
| Popis: |
Antigen-specific, MHC-restricted αβ T cells are necessary for protective immunity against Mycobacterium tuberculosis, but the ability to broadly study these responses has been limited. In the present study, we used single-cell and bulk T cell receptor (TCR) sequencing and the GLIPH2 algorithm to analyze M. tuberculosis-specific sequences in two longitudinal cohorts, comprising 166 individuals with M. tuberculosis infection who progressed to either tuberculosis (n = 48) or controlled infection (n = 118). We found 24 T cell groups with similar TCR-β sequences, predicted by GLIPH2 to have common TCR specificities, which were associated with control of infection (n = 17), and others that were associated with progression to disease (n = 7). Using a genome-wide M. tuberculosis antigen screen, we identified peptides targeted by T cell similarity groups enriched either in controllers or in progressors. We propose that antigens recognized by T cell similarity groups associated with control of infection can be considered as high-priority targets for future vaccine development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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