| Autor: |
Yinping, Meng, Rong, Fu, Hui, Liu, Yihao, Wang, Lijuan, Li, Chunyan, Liu, Tian, Zhang, Shaoxue, Ding, Liyan, Li, Erbao, Ruan, Wen, Qu, Huaquan, Wang, Xiaoming, Wang, Guojin, Wang, Hong, Liu, Yuhong, Wu, Jia, Song, Limin, Xing, Jing, Guan, Zonghong, Shao |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Chinese Journal of Hematology |
| ISSN: |
0253-2727 |
| Popis: |
To explore the expression levels of terminal complement complex (C5b-9) and CD62p on platelets and the soluble C5b-9 (sC5b-9) level in serum in patients with PNH or PNH-aplastic anemia (AA).Serum levels of sC5b-9, complement C3 and C4 were detected by using ELISA in 25 patients with PNH/PNH-AA. The quantities of C5b-9 and CD62p on the membrane of platelets were detected by flow cytometry.①In PNH/PNH-AA group, the serum sC5b-9 level [390.27(265.73-676.87) μg/L] was lower than that in control group [540.39(344.20-1 576.78) μg/L] (P0.01). ②The platelet PNH clone (CD59⁻CD61⁺/CD61⁺) size [50.58(23.29-81.60)%] was bigger in the PNH/PNH-AA group than that [23.57(15.58-29.02)%] in control group (P0.01). The percentages of C5b-9 deposition (C5b-9⁺CD61⁺/CD61⁺) were higher on the PNH clone platelets (CD59⁻CD61⁺) in the PNH/PNH-AA group [(17.53 ± 6.27)%] than those on the normal platelets (CD59⁺CD61⁺) in PNH patients 11.33±5.03）%］ and control [(10.88±3.58)%] group (P0.01). ③ The expression of CD62p (CD62p⁺CD61⁺/CD61⁺) on PNH clone platelets in PNH patients [(61.98 ± 11.71)%] was higher than that on the normal platelets in PNH patients [(43.76±11.30)%] and control group [(38.23±18.07)%] (P0.01). In addition, the expression of CD62p on normal platelets was higher in PNH patients than control (P0.05). ④The deposition of C5b-9 positively correlated with the expression of CD62p on the platelets (r=0.559, P=0.002).Deficiency of CD59 antigen on platelets in PNH patients may lead to the deposition of C5b-9 on its membrane and its dysfunction, which may contribute to thrombosis events in PNH. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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