| Autor: |
N Akbari, Oghaz, S, Hatamzadeh, K, Rahnama, M Khorrami, Moghaddam, S, Vaziee, Z, Tazik |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
World journal of microbiologybiotechnology. 38(10) |
| ISSN: |
1573-0972 |
| Popis: |
Cladosporium spp. are among the most important plant pathogens, plant endophytes, insect parasites and human pathogens in nature. The aim of this study was to increase the speed and accuracy of Cladosporium spp. spore counting using UV-visible spectrophotometry based on the regression model in a water suspension. Spores of C. ramotenellum AM55, C. limoniforme Br15, C. tenuissimum K15 and C. cladosporioides Ld13 fungi were diluted in sterile distilled water several times. Spore concentration/ml (SC) was counted with a hemocytometer. The spectrophotometer visible light absorption (ABS) was measured under 14 wavelengths from 300 to 950 nm for each dilution. The results showed that the morphological variation of the spores greatly affect the determination of the suitable wavelength. 650, 750, 500 and 400 nm wavelengths had the highest coefficient of determination (R |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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