| Autor: |
D L, Greiner, L D, Shultz, D, Deluca, J H, Leif, S W, Christianson, R M, Hesselton |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
In vivo (Athens, Greece). 10(1) |
| ISSN: |
0258-851X |
| Popis: |
Human cord blood (HuCB) can colonize a murine fetal thymus organ culture (FTOC) and generate phenotypically immature (CD4+ CD8+) and mature (CD4+ CD8-; CD4- CD8+) T cells. We have used this model system to demonstrate that the human T cells that develop in this culture system can be infected with HIV-1. A cytopathic and non-cytopathic patient isolate of HIV-1 were used to infect FTOC established using C.B-17 or NOD/LtSz.scid/scid strain fetal thymic lobes colonized with HuCB. At 13-15 days after infection, FTOC were placed in co-culture with human PHA-blasts. These co-cultures demonstrated the presence of replicating HIV-1. Few human CD45+ cells were detectable in the thymic lobes that were infected with HIV-1, while high numbers of human CD45+ T cells were present in the uninfected cultures. These results demonstrate the cytopathicity of HIV-1 on human T lymphocytes that have developed in a HuCB colonized FTOC system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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