Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II)
| Autor: | N H, Ing, J M, Beekman, S Y, Tsai, M J, Tsai, B W, O'Malley |
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| Rok vydání: | 1992 |
| Předmět: |
Cell Nucleus
Transcriptional Activation Receptors Steroid Base Sequence Transcription Genetic Recombinant Fusion Proteins Molecular Sequence Data Restriction Mapping Polymerase Chain Reaction Recombinant Proteins Models Structural Oligodeoxyribonucleotides Receptors Estrogen Transcription Factor TFIIB Humans Electrophoresis Polyacrylamide Gel Amino Acid Sequence Cloning Molecular Glutathione Transferase HeLa Cells Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 267(25) |
| ISSN: | 0021-9258 |
| Popis: | The S300-II factor was discovered as an activator of ovalbumin gene transcription with the chicken ovalbumin upstream promoter-transcription factor (COUP-TF, Sagami, I., Tsai, S. Y., Wang, H., Tsai, M.-J., and O'Malley, B. W. (1986) Mol. Cell. Biol. 6, 4259-4267). Although S300-II does not bind DNA selectively, it stabilizes the binding of COUP-TF to its ciselement (Tsai, S. Y., Sagami, I., Wang, H., Tsai, M.-J., and O'Malley, B. W. (1987) Cell 50, 701-709). Purified S300-II is also required for steroid receptor-activated transcription. Cloning and sequencing of S300-II showed that it is the general transcription factor TFIIB. Specific protein-protein interactions between recombinant S300-II/TFIIB and three members of the steroid hormone receptor superfamily, COUP-TF, estrogen receptor, and progesterone receptor, indicate that S300-II/TFIIB is one of the targets of these transactivators. Interestingly, a truncated estrogen receptor construct containing only the N-terminal transcription activation function 1 did not interact with S300-II/TFIIB in our assay, revealing that individual transcription activation functions of a single steroid hormone receptor may contact different targets. Demonstration of a direct association of S300-II/TFIIB and COUP-TF, independent of additional "adaptor" proteins, suggests that members of the steroid hromone receptor superfamily facilitate the transcription of activated genes at least in part via protein-protein interactions with the general transcription factor TFIIB. |
| Databáze: | OpenAIRE |
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