The CD8+ granzyme B+ T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells

Autor:	P C, Wever, H J, Van Der Vliet, L H, Spaeny, A M, Wolbink, F N, Van Diepen, C J, Froelich, C E, Hack, I J, ten Berge
Rok vydání:	1998
Předmět:	Serine Endopeptidases Antibodies Monoclonal Apoptosis CD8-Positive T-Lymphocytes Flow Cytometry Lymphocyte Activation Immunohistochemistry Granzymes Lymphocyte Subsets Humans fas Receptor hormones hormone substitutes and hormone antagonists Cells Cultured Research Article
Zdroj:	Immunology. 93(3)
ISSN:	0019-2805
Popis:	Granzyme B (GrB) has been implicated in induction of apoptosis in target cells. The presence of GrB in peripheral blood CD8+ T cells from healthy individuals was analysed in immunocytochemical and flow cytometric studies. Furthermore, CD8+ GrB- T cells and CD8+ GrB+ T cells were compared regarding phenotypical characteristics and susceptibility to both spontaneous and Fasmediated apoptosis. GrB was expressed by approximately one-fifth of CD8+ T cells. Compared with the CD8+ GrB- T-cell subset, the CD8+ GrB+ T-cell subset contained cells that were relatively more activated and more prone to spontaneous apoptosis. Culturing of cells with immunoglobulin M (IgM) anti-Fas monoclonal antibody had no additional effect on the number of CD8+ GrB+ T cells undergoing apoptosis. We suggest that the presence of CD8+ GrB+ T cells in peripheral blood from healthy individuals results from immune surveillance or contact with infectious agents, and that spontaneous apoptosis of these cells might serve as a mechanism for their eventual clearance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::859d8bf8a0327c73de3cf535b0403916 https://pubmed.ncbi.nlm.nih.gov/9640249 Zobrazit plný text záznamu Plný text