| Autor: |
Thomas, Keaney, Louise, O'Connor, Janusz, Krawczyk, Moutaz A, Abdelrahman, Amjad H, Hayat, Margaret, Murray, Michael, O'Dwyer, Melanie, Percy, Stehpen, Langabeer, Karl, Haslam, Barry, Glynn, Ciara, Mullen, Evelyn, Keady, Sinéad, Lahiff, Terry J, Smith |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Journal of clinical pathology. 70(8) |
| ISSN: |
1472-4146 |
| Popis: |
Somatic insertions/deletions in exon 9 of the calreticulin gene have been identified in patients with essential thrombocythemia and primary myelofibrosis. Over 55 mutations have been discovered, 80% of which consist of either type 1 52-bp deletion or type 2 5-bp insertion. Other mutations (types 3-5) in conjunction with types 1 and 2 account for87% of identified mutations. The aim of this study was development of a rapid PCR-based assay using LightCycler Hybridisation Probes for the detection of type 1-5A real-time PCR assay using a novel HybProbe set was developed for use on the LightCycler 480 Instrument II. The acceptor probe was labelled with LC640 and Faststart DNA Master HybProbe kit was used for PCR reactions.Assay limit of detection was determined to be seven target copies with a probability of 95%. The specificity of the assay was determined by using synthetic constructs ofThe novel assay described has potential for application as a rapid, sensitive, high-throughput screening method in the clinical diagnostics setting. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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