Drug resistance and P-glycoprotein expression in endothelial cells of newly formed capillaries induced by tumors
| Autor: | M, Iwahana, N, Utoguchi, T, Mayumi, M, Goryo, K, Okada |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Mice Inbred ICR Umbilical Veins Neovascularization Pathologic Paclitaxel Blotting Western Calcium Channel Blockers Antineoplastic Agents Phytogenic Rats Mice Verapamil Drug Resistance Neoplasm Antineoplastic Combined Chemotherapy Protocols Animals Humans ATP Binding Cassette Transporter Subfamily B Member 1 Endothelium Vascular Sarcoma 180 Cell Division Cells Cultured |
| Zdroj: | Anticancer research. 18(4C) |
| ISSN: | 0250-7005 |
| Popis: | The inhibitory effects of vincristine (VCR) with or without verapamil (VER) on angiogenesis in mouse subcutaneous fascia induced by mouse sarcoma 180 cells were assessed using the dorsal air sac method. VCR combined with VER had inhibitory effects on tumor-induced angiogenesis, but VCR alone did not inhibit capillarization. The chemosensitivity of human umbilical vein endothelial cells (HUVEC) and tumor-derived endothelial cells from rat KMT-17 fibrosarcoma (TEC) was examined using the microculture tetrazolium assay. VCR and taxol had strong anti-proliferative activity against HUVEC, but only weakly inhibited the proliferation of TEC. In combination with VER, VCR and Taxol inhibited the proliferation of TEC. Cisplatin, mitomycin C, and 5-fluorouracil had weakly anti-proliferative activity against both HUVEC and TEC. Expression of P-glycoprotein (P-gp) was found in TEC, but not in HUVEC using western blot analysis. These findings indicate that drug resistance and P-gp expression appeared on newly formed capillaries induced by rodents tumors. |
| Databáze: | OpenAIRE |
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