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To identify the difficult antibody specificity of 1 PNH patient with blood transfusion history by genotyping.RH typing of this patient was performed using gel card method, the antibody specificity was identified by panel cells, the RH-unrelated phenotype were excluded by genotyping method in difficult condition of serologic identification, furthmore different RH phenotype cells were used for adsorption-elution so as to re-examine the reactivity of antibodies in this patient's serum, and finally different RH phenotype cells were combined to exclude other unrelated antibodies.The RH phenotype presented as double population for C antigen, and positive agglutination for the other antigens. The results of RHD zygote, together with RHD and RHCE sequencing showed that the RHD genotype was homozygous RHD/RHD, and the c.122A＞G mutation did not found in RHCE gene, thus CGenotyping can be auxiliarily applied to the identification of difficult antibodies in serum of a patient, thereby reducing the risk of blood transfusion.基因分型技术对1例PNH患者抗-C同种抗体合并抗-f类同种自身抗体的辅助鉴定.通过基因分型对1例有输血史的PNH患者疑难抗体进行鉴定.采用卡式凝胶法检测患者RH分型, 谱细胞鉴定PNH患者抗体特异性。在血清学鉴定困难的情况下, 利用基因分型的方法排除RH无关表型, 利用不同的RH表型细胞分别吸收放散后进行抗体鉴定, 不同的RH表型细胞组合排除其它无关抗体.卡式凝胶法鉴定显示RH分型C抗原存在双群, 其它抗原阳性, 分别进行RHD合子型鉴定、RHD和RHCE基因测序分析。结果显示, RHD基因型为纯合子RHD/RHD, RHCE基因未发现c.122A＞G突变, 排除携带有C基因分型可以辅助用于患者血清中疑难抗体的鉴定, 从而减少患者输血风险. |
