Lack of acidic fibroblast growth factor activation by heparan sulfate species from diabetic rat skin

Autor:	M C, Bourin
Rok vydání:	1997
Předmět:	Male Liver Animals Fibroblast Growth Factor 1 Fibroblast Growth Factor 2 Heparitin Sulfate Chromatography Affinity Streptozocin Amidohydrolases Cell Line Diabetes Mellitus Experimental Rats Skin
Zdroj:	Glycoconjugate journal. 14(4)
ISSN:	0282-0080
Popis:	The glucosaminoglycans isolated from the skin of control and streptozotocin-diabetic rats were fractionated on ion-exchange chromatography into a heparan sulfate (HS)-like and a heparin-like species. In addition, a low sulfated fraction was isolated from the diabetics. The HS and heparin-like fractions isolated from the diabetics (in contrast to the low sulfated fractions) retained high affinity for the acidic (FGF-1) and basic (FGF-2) fibroblast growth factors. In culture, the fractions purified from the control rats and the heparin-like material isolated from the diabetics mediated the biological activity of both FGFs in a dose-dependent manner. By contrast, the diabetic HS-like fractions promoted the biological activity of FGF-2 but not of FGF-1. The results support the idea that the structural motives in HS required for FGF-1 and FGF-2 mediated receptor signalling are different. They may be relevant to the impaired wound healing observed in the disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::83d7a62bb0e49b10b32deb31a9030bd9 https://pubmed.ncbi.nlm.nih.gov/9249139 Zobrazit plný text záznamu Full text from SpringerLink