| Autor: |
Alvin C H, Ma, Xiangguo, Shi, Bai-Liang, He, Yuhan, Guo, Anskar Y H, Leung |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1633 |
| ISSN: |
1940-6029 |
| Popis: |
The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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