| Autor: |
A V, Shugaliĭ, D I, Chernyĭ, M V, Lichina, A V, German, A A, Aleksandrov |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia biologiia. 16(5) |
| ISSN: |
0026-8984 |
| Popis: |
On the basis of the reaction of dibromoethylacetate with adenine and cytosine at the uncoiled regions of DNA it was possible to fix these molten regions (the degree of denaturation was about 16%) for rat liver mitochondrial DNA. Modification of all accessible adenine and cytosine residues in 0.14 M Na-acetate, pH 5.65, was completed at 70-80 degrees C in 15-20 min. Using the computer orientation for the set of fixed BamHI-fragments and the linear molecules of full length the denaturation map of mtDNA was constructed, the GC-content of the molten regions was about 28%. The dibromoethylacetate is a perspective agent for screening DNA with a low content of destabilizing regions (for instance, DNA of malignant cells). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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