NMR experiments for the sign determination of homonuclear scalar and residual dipolar couplings

Autor:	G, Otting, M, Rückert, M H, Levitt, A, Moshref
Rok vydání:	2000
Předmět:	Carbon Isotopes Magnetics Aprotinin Models Chemical Nitrogen Isotopes Gels Nuclear Magnetic Resonance Biomolecular
Zdroj:	Journal of biomolecular NMR. 16(4)
ISSN:	0925-2738
Popis:	A modified version of the JHH-TOCSY experiment, 'signed COSY', is presented that allows the determination of the sign of residual dipolar 1H-1H coupling constants with respect to the sign of one-bond 1H-X coupling constants in linear three-spin systems X-1H-1H, where X = 13C or 15N. In contrast to the original JHH-TOCSY experiments, the signs of J(HH) couplings may be determined for CH2-CH2 moieties and for uniformly 13C/15N-labelled samples. In addition, sensitivity is enhanced, diagonal peaks are suppressed and cross peaks are observed only between directly coupled protons, as in a COSY spectrum.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8398ad9cca9f26c0f0c3ba8ac01c149c https://pubmed.ncbi.nlm.nih.gov/10826886 Zobrazit plný text záznamu Full text from SpringerLink