| Autor: |
O A, Rykovskaia, L M, Smolikova, E V, Monakhova, O S, Chemisova, O A, Podoĭnitsyna, E N, Golenishcheva, E M, Sanamiants, M M, Sagakiants, R R, Dalikova |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (6) |
| ISSN: |
0372-9311 |
| Popis: |
Formation of Vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology.Traditional biochemical tests and PCR-testing of species-specific genes were used to confirm species membership. Catalase, DNAse, proteolytic and tweenase activity was determined by common methods. Virulence was evaluated by a complex method: hemolytic activity was determined in Kanagawa test (KT), urease--in Christensen medium, PRC-testing of tdh and-trh genes. Serotyping was carried out with a commercial O/K-sera kit. PCR-genotyping was carried out by marker genes of 7 pathogenicity islands (VPaI-1-7).Species membership was confirmed for the studied strains. Serologic typing allowed to detect members of 18 serologic groups among the collection strains. All the collection cultures were divided into 4 groups based on KT-Ure-tdh-trh features recombination. A number of genetic variants were detected, strains belonging to a pandemic group and O3:K6 serogroup were determined.A collection of V. parahaemolyticus cultures was formed and characterized by a large set of pheno- and genotypic features. A database was developed including information on strain origins, pheno- and genetic features, with genetic variants given, for ease of use of the collection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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