Characterization of two newly established cell lines derived from squamous cell carcinomas of the oesophagus
| Autor: | M, Sarbia, N, Bösing, B, Hildebrandt, P, Koldovsky, C D, Gerharz, H E, Gabbert |
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| Rok vydání: | 1997 |
| Předmět: |
Esophageal Neoplasms
Cell Cycle Transplantation Heterologous Cell Culture Techniques Mice Nude Desmosomes Immunohistochemistry Cell Line Chromosome Banding Mice Microscopy Electron Karyotyping Carcinoma Squamous Cell Tumor Cells Cultured Animals Humans Keratins Vimentin Microscopy Phase-Contrast Metaphase |
| Zdroj: | Anticancer research. 17(3C) |
| ISSN: | 0250-7005 |
| Popis: | This study describes characteristics of two newly established cell lines (OSC-1 and OSC-2), derived from two oesophageal squamous cell carcinomas. Morphologically, OSC-1 cells and OSC-2 cells grew in epithelial cobblestone patterns with cells piling up to 4 cells. Ultrastructurally, both cell lines showed formation of desmosomes; however, tonofilaments were only formed by OSC-2 cells. Immunohistochemical investigations revealed coexpression of vimentin and cytokeratin in OSC-1 cells and OSC-2 cells. A cytokeratin subtype typical for mature squamous epithelia (cytokeratin 13) was expressed only in OSC-2 cells. OSC-1 cells showed tumour formation in nude mice, whereas OSC-2 cells did not. Cytogenetic analysis revealed that OSC-1 cells had a hyperdiploid karyotype and OSC-2 cells had a near-triploid karyotype. In both cell lines, the formation of multicellular spheroids could be induced. In conclusion, in comparison with OSC-2 cells the OSC-1 cells were characterized by a poorer degree of differentiation and by a more aggressive growth behaviour in vivo. |
| Databáze: | OpenAIRE |
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