Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm

Autor: Ching-Fu, Tu, Shu-Hui, Peng, Chin-Kai, Chuang, Chi-Hong, Wong, Tien-Shuh, Yang
Rok vydání: 2022
Zdroj: Animal bioscience.
ISSN: 2765-0189
Popis: Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells (iPSCs), somatic cell nuclear transfer (SCNT), cryopreservation, and nonsurgical embryo transfer (NSET). The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.
Databáze: OpenAIRE