[Porcine circovirus type 2 induces apoptosis by exosomal miR-125a-5p targeting

Autor: Dianning, Duan, Huawei, Shen, Yanmin, Pan, Rui, Feng, Lin, Zhang, Lingguang, Lu, Jiankui, Liu, Longxin, Qiu, Hongbo, Chen
Rok vydání: 2022
Předmět:
Zdroj: Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 38(8)
ISSN: 1872-2075
Popis: In order to investigate the apoptosis triggered by porcine circovirus type 2 (PCV2) in lymphocytes and the underlying mechanism, the levels of apoptosis and the expression levels of miRNA were examined by flow cytometry, Western blotting and real-time PCR (qPCR). The mimics or inhibitors of miR-125a-5p, an apoptosis-related miRNA, were transfected into PK-15 cells, and the apoptosis rate was examined upon overexpression or inhibition of mir-125a-5p. The target gene of mir-125a-5p was predicted by bioinformatics method, and the regulation of mir-125a-5p on the target gene was analyzed by luciferase reporter assay. The expressions of Bcl-2, Bax, cytochrome C and caspase-3 were detected by Western blotting. The results showed that exosomes secreted by PK-15 cells infected with PCV2 significantly increased the lymphocyte apoptosis rate, which was dose-dependent in certain concentration range. The expression of miR-125a-5p was dramatically increased. The apoptosis rate was increased significantly in the cells transfected with miR-125a-5p. It was predicted that there were binding sites of miR-125a-5p at
Databáze: OpenAIRE