Stimulation of neuronal cells by culture supernatant of T lymphocytes triggered by anti-CD3 mAb followed by propagation in the presence of interleukin-2
| Autor: | Masae, Ishiguro, Alan, Okada, Kiyofumi, Asai, Kiyohide, Kojima, Hidechika, Okada |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Neurons Neurogenesis Amyotrophic Lateral Sclerosis Antibodies Monoclonal Brain Granulocyte-Macrophage Colony-Stimulating Factor Flow Cytometry Immunotherapy Adoptive Rats Up-Regulation Rats Sprague-Dawley Alzheimer Disease Animals Cytokines Humans Interleukin-2 Rats Wistar Killer Cells Lymphokine-Activated Cell Proliferation T-Lymphocytes Cytotoxic |
| Zdroj: | Microbiology and immunology. 60(1) |
| ISSN: | 1348-0421 |
| Popis: | Performance status (PS) frequently improves occurs in cancer patients who have been infused with their own lymphokine-activated killer T cells (LAK-T). In the present study, a culture supernatant of LAK-T (LAK-T sup) administered to 8-week-old rats caused neurogenesis as evidenced by increased 5-ethynyl-2'-deoxyuridine staining of brain tissues. Intravenous injection of granulocyte-macrophage colony stimulating factor (GM-CSF), a major cytokine in LAK-T sup, had a similar effect. Furthermore, LAK-T sup induced Ca(++) increase in rat hippocampal brain slices that was detected in neuronal cells by emission of Fluo-8 NW at 520 nm. The same effect was observed with an rGM-CSF solution. GM-CSF may activate neuronal cells by stimulating the glial cells that surround and attach to them. If so, GM-CSF and LAK-T sup may improve the motor neurons of patients with amyotrophic lateral sclerosis. The neurogenerative effect of GM-CSF in LAK-T sup may also help improve brain function in aged adults including those with dementia such as Alzheimer's disease. |
| Databáze: | OpenAIRE |
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