An Arabidopsis thaliana G-box-binding protein similar to the wheat leucine zipper protein identified as HBP-1
| Autor: | U, Schindler, J R, Ecker, A R, Cashmore |
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| Rok vydání: | 1991 |
| Předmět: |
Leucine Zippers
Binding Sites Sequence Homology Amino Acid Molecular Sequence Data Arabidopsis Nuclear Proteins DNA DNA-Binding Proteins Blotting Southern Basic-Leucine Zipper Transcription Factors G-Box Binding Factors Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Plant Proteins Transcription Factors |
| Zdroj: | Symposia of the Society for Experimental Biology. 45 |
| ISSN: | 0081-1386 |
| Popis: | G-box (CCACGTGG) like sequences are present in a variety of plant promoters and in many cases they have been demonstrated to be required for maximal expression of the corresponding gene. A nuclear protein, GBF, interacts specifically with the G-box motif of several RBCS and CAB promoters. Here we describe the isolation of a cDNA from Arabidopsis thaliana that encodes a protein, designated GBF-1, with DNA binding properties similar to GBF. GBF-1 is characterized by a basic/leucine zipper motif which is strikingly similar to the wheat protein identified as HBP-1. GBF-1 also interacts with an oligonucleotide derived from the wheat histone 3 promoter containing the binding site (hexamer, TGACGT) for HBP-1. This DNA element also contains a G-box-like motif, modification of which results in loss in binding of GBF-1. |
| Databáze: | OpenAIRE |
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