| Autor: |
Pascale, Mariani, Marick, Lae, Armelle, Degeorges, Wulfran, Cacheux, Emmanuelle, Lappartient, Audrey, Margogne, Jean-Yves, Pierga, Véronique, Girre, Laurent, Mignot, Marie Christine, Falcou, Rémy-Jacques, Salmon, Olivier, Delattre, Patricia, De Cremoux |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Anticancer research. 30(10) |
| ISSN: |
1791-7530 |
| Popis: |
KRAS somatic mutations are the main predictive factor for non response to EGFR-targeted monoclonal antibodies in metastatic colorectal cancer (mCRC) patients. We compared KRAS mutational status in the primary tumour and the corresponding metastases (1 to 4 sites) in 38 mCRC patients. KRAS mutational status was analysed using direct sequencing, SNAPShot multiplex PCR and Scorpion Taqman PCR analysis. Results showed 54% of primary tumours had KRAS mutations. A concordance of 97% between primaries and metastatic sites was observed. A tumour heterogeneity was also demonstrated in 5% of mCRC. One case with three different primary tumours harboured three different KRAS mutations, and only one was represented in the unique metastasis of this patient. We concluded there was a high concordance in the KRAS status between the primary tumour and metastases. More than one informative block and more sensitive assay may increase the accuracy of KRAS status determination. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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