The three-dimensional structure of 'Lonely Guy' from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins

Autor:	Lenka, Dzurová, Federico, Forneris, Simone, Savino, Petr, Galuszka, Josef, Vrabka, Ivo, Frébort
Rok vydání:	2015
Předmět:	Fungal Proteins Models Molecular Cytokinins Aminohydrolases Carboxy-Lyases Amino Acid Sequence Claviceps
Zdroj:	Proteins. 83(8)
ISSN:	1097-0134
Popis:	The recently discovered cytokinin (CK)-specific phosphoribohydrolase "Lonely Guy" (LOG) is a key enzyme of CK biosynthesis, converting inactive CK nucleotides into biologically active free bases. We have determined the crystal structures of LOG from Claviceps purpurea (cpLOG) and its complex with the enzymatic product phosphoribose. The structures reveal a dimeric arrangement of Rossmann folds, with the ligands bound to large pockets at the interface between cpLOG monomers. Structural comparisons highlight the homology of cpLOG to putative lysine decarboxylases. Extended sequence analysis enabled identification of a distinguishing LOG sequence signature. Taken together, our data suggest phosphoribohydrolase activity for several proteins of unknown function.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8166e3dbfe370fff569f39208df02825 https://pubmed.ncbi.nlm.nih.gov/26010010 Zobrazit plný text záznamu Plný text