Structural characterization of anti-UV components from Sasa senanensis Rehder extract
| Autor: | Tomohiko, Matsuta, Hiroshi, Sakagami, Tomoko, Sugiura, Madoka, Kitajima, Hiroshi, Oizumi, Takaaki, Oizumi |
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| Rok vydání: | 2012 |
| Předmět: |
Magnetic Resonance Spectroscopy
Coumaric Acids Dose-Response Relationship Drug Ethanol Molecular Structure Cell Survival Plant Extracts Ultraviolet Rays Biphenyl Compounds Radiation-Protective Agents Free Radical Scavengers Lignin Antioxidants Plant Leaves Picrates Cell Line Tumor Humans Spectrophotometry Ultraviolet Propionates Sasa Oxidation-Reduction Chromatography High Pressure Liquid |
| Zdroj: | In vivo (Athens, Greece). 27(1) |
| ISSN: | 1791-7549 |
| Popis: | Previous studies have shown antiviral, antibacterial and anti-inflammatory activity of alkaline extract of the leaves of Sasa senanensis Rehder (SE). However, active components have not been identified. We isolated the substances that exhibit anti-UV activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity from SE and estimated their putative structures.The anti-UV substances (SEE-1 and SEE-2) were isolated from SE by ethanolic extraction, Wakosil chromatography and recycled high-performance liquid chromatography (HPLC) at a yield of 0.22 and 0.18%, respectively. The structural analysis was carried out with (1)H-nuclear magnetic resonance (NMR), (13)C-NMR and UV absorption.SEE-1 exhibited approximately four-fold higher anti-UV activity and slightly lower DPPH radical-scavenging activity, compared to SE. SEE-1 was identified as p-coumaric acid derivative(s), a lignin precursor.The present study demonstrated for the first time the presence of lignin precursors in SE, which may explain why SE exhibits many of the properties of lignin-carbohydrate complexes. |
| Databáze: | OpenAIRE |
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