Autoantibodies directed against CD43 molecules with an altered glycosylation status on human immunodeficiency virus type 1 (HIV-1)-infected CEM cells are found in all HIV-1+ individuals
| Autor: | V, Giordanengo, M, Limouse, L, Desroys du Roure, J, Cottalorda, A, Doglio, A, Passeron, J G, Fuzibet, J C, Lefebvre |
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| Rok vydání: | 1995 |
| Předmět: |
Acquired Immunodeficiency Syndrome
Glycosylation Leukosialin Sialoglycoproteins T-Lymphocytes HIV Infections Cross Reactions Hepatitis B Autoantigens N-Acetylneuraminic Acid Cell Line Arthritis Rheumatoid Molecular Weight Immunoglobulin M Antigens CD Immunoglobulin G HIV-1 Sialic Acids Humans Lupus Erythematosus Systemic Autoantibodies |
| Zdroj: | Blood. 86(6) |
| ISSN: | 0006-4971 |
| Popis: | Autoantibodies to lymphocytes have been detected in sera from human immunodeficiency virus type 1 (HIV-1)-infected individuals, and several autoantigens have been described. Among them, hyposialylated CD43 has been shown to be a target for autoantibodies in up to 47% of HIV+ individuals. However, the corresponding autoantigen (ie, the incompletely sialylated CD43) has not been isolated from blood cells of HIV-1-infected individuals. Recently, we have observed in vitro that HIV-1 productively or latently infected CEM cells (CEMLAI/NP) express CD43 molecules with modified glycosylation (mogly CD43). Using CEMLAI/NP cells, which do not express any structural viral antigen, we show now that all of the tested HIV+ sera from asymptomatic individuals, and up to 86% of those from subjects at the acquired immunodeficiency syndrome stage contain antibodies (mainly IgM and, to a lesser degree, IgG) that recognize the surface of CEMLAI/NP cells, and precipitate mogly CD43 molecules from the cells lysates. Taken together with our previous demonstration of altered glycosylation of CD43 from HIV-1-infected CEM cells in vitro, the constant antimogly CD43 autoimmune response observed from asymptomatic HIV-1+ subjects is likely to illustrate the occurrence of an altered glycosylation in vivo of the major lymphocyte surface CD43 glycoprotein, associated with HIV-1 infection. |
| Databáze: | OpenAIRE |
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