[The study on reversing mechanism of multidrug resistance of K562/A02 cell line by curcumin and erythromycin]
| Autor: | Hong-yu, Chang, Kai-li, Pan, Fu-cheng, Ma, Xi-ying, Jiao, Hua-feng, Zhu, Jian-hong, Liu, Ying, Huang, Yu-hong, Cao |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Curcumin
Time Factors Cell Survival Reverse Transcriptase Polymerase Chain Reaction Antineoplastic Agents Drug Synergism Immunohistochemistry Drug Resistance Multiple Erythromycin Gene Expression Regulation Neoplastic Drug Resistance Neoplasm Humans ATP Binding Cassette Transporter Subfamily B Member 1 Leukemia Erythroblastic Acute RNA Messenger K562 Cells Cell Proliferation Epirubicin |
| Zdroj: | Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi. 27(4) |
| ISSN: | 0253-2727 |
| Popis: | To investigate the effects of curcumin (Cur) and erythromycin (EM) on multidrug resistance (MDR) reversal of K562/A02 cell line and their mechanism.MTT assay was employed to determine the sensitivity of Cur, EM-treated K562/A02 cells to adriamycin (ADM). Flow cytometry was used to measure intracellular mean fluorescence intensity (MFI) of daunorubicin (DNR). P-gp expression was determined by immunohistochemistry. RT-PCR technique was used to examine the mdr1 mRNA level.IC(50) of ADM in K562/A02 cells was decreased when treated with Cur or EM, and the reversal times (RvT) was 4.9, 3.7 respectively. The RvT reached to 11.3 when treated with Cur (2.5 microg/ml) combined with EM (120 microg/ml). The DNR MFI in K562/A02 cells was significantly lower than that in K562 cells (P0.01), and was increased significantly when treated with Cur (2.5 microg/ml) or EM (120 microg/ml) (P0.05). There was no significant difference between DNR MFI of K562/A02 cells treated with Cur (2.5 microg/ml) or EM (120 microg/ml). Immunohistochemistry showed that P-gp expression was significantly higher in K562/A02 cells than in K562 cells (P0.01), and was reduced in K562/A02 cells treated with each (P0.01), though being still higher than that in K562 cells (P0.01). P-gp expression of K562/A02 cells treated with each drug for 5 days were lower than that for 3 days (P0.01), and lowered further when treated with Cur and EM together (P0.01). Mdr1 mRNA level in K562/A02 cells was higher than in K562 cells (P0.01), and was decreased when treated with each of the drugs (P0.01). The mdr1 mRNA level of K562/A02 cells treated with Cur (2.5 microg/ml) plus EM (120 microg/ml) was decreased most significantly than that treated with other group of drugs. After 5 day treatment the mdr1 mRNA level of K562/A02 cells with Cur (2.5 microg/ml) was lower than that with EM 120 microg/ml (P0.01).Either Cur or EM can partly reverse the multidrug resistance of K562/A02 cells and decrease the expression and function of P-gp in a time-dependent way. MDR reversing effect of Cur combined with EM is stronger than that of Cur or EM alone. |
| Databáze: | OpenAIRE |
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