[Analysis of aromatic hydrocarbon catabolic genes in strains isolated from soil in Patagonia]
| Autor: | G S, Vacca, B, Kiesel, L, Wünsche, O H, Pucci |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Argentina Naphthalenes Protocatechuate-3 4-Dioxygenase Hydrocarbons Aromatic Polymerase Chain Reaction Catechol 1 2-Dioxygenase Catechol 2 3-Dioxygenase Dioxygenases Biodegradation Environmental Bacterial Proteins Genes Bacterial Enzyme Induction Pseudomonas Oxygenases Soil Pollutants Soil Microbiology |
| Zdroj: | Revista Argentina de microbiologia. 34(3) |
| ISSN: | 0325-7541 |
| Popis: | Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia. |
| Databáze: | OpenAIRE |
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