| Autor: |
Ning, Song, Ai-jing, Liu, Xue-feng, Gao, Xian-li, Guo, Ji-tao, Guan, Jian-ling, Wu, Rui-fang, Zhang, Lin, Duan, Wen-shu, He |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Zhonghua nei ke za zhi. 52(10) |
| ISSN: |
0578-1426 |
| Popis: |
To investigate the effect of selective phosphodiesterase (PDE) 4 inhibitors on nuclear factor kappa B (NF-κB), tumor necrosis factor-α (TNFα) and interleukin-8 (IL-8) secreted by peripheral blood mononuclear cells (PBMCs) in patients diagnosed as rheumatoid arthritis with interstitial lung disease (RA-ILD).PBMCs isolated from 15 healthy volunteers (group A) and 20 patients with untreated active RA-ILD (group B) were cultured in vitro. PBMCs from healthy subjects were considered as normal control. PBMCs from RA-ILD patients were divided into four groups with different treatment: blank group (B1), theophylline group (B2), selective PDE4 inhibitor rolipram group (B3), and glucocorticoid group (B4) with dexamethasone. The expression of NF-κB was determined by immunocytochemical staining, and the levels of TNFα and IL-8 in the culture supernatant were detected by enzyme linked immunosorbent assay (ELISA).(1) The activity of NF-κB and the levels of TNFα and IL-8 in group B1 were significant higher than that in group A (P0.01). Compared with group B1, three parameters above were similar to those in group B2 (P0.05), while group B3 and group B4 had significant decreased levels of three parameters (P0.01); IL-8 level in group B4 was significantly lower than that in group B3 (P0.05). (2) TNFα and IL-8 levels were positively correlated with NF-κB activity in group B (r = 0.902 and 0.735, P0.01 respectively). (3) The reduction of TNFα and IL-8 levels were positively correlated with reduction of NF-κB activity after intervention of rolipram in group B3 (r = 0.874, P0.01; r = 0.561, P0.05 respectively).NF-κB activation and proinflammatory cytokines were involved in the pathogenesis of RA-ILD. selective PDE4 inhibitors may inhibit the production of inflammatory cytokines by inhibiting the activity of the transcription factor NF-κB in PBMC, thus inhibiting the inflammatory reaction of RA-ILD. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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