| Autor: |
D P, McManus, E, Garcia-Zepeda, A, Reid, A K, Rishi, A, Flisser |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
Acta Leidensia. 57(2) |
| ISSN: |
0065-1362 |
| Popis: |
The construction and antibody screening of Taenia solium cDNA libraries, generated in the Escherichia coli bacteriophage lambda gt11, with the identification of clones putatively expressing antigen B, T. solium-specific and other antigens is described. Lysogens were produced from a number of selected clones and beta-galactosidase fusion peptides ranging in Mr of approximately 135,000-150,000 were demonstrated. These proteins were shown by immunoblotting to be reactive with a pool of sera from cysticercotic patients originally used in the cDNA library screening. We report a method whereby Taenia (T. saginata and T. pisiformis) eggs can be detected with high sensitivity in a specific DNA dot-blot hybridisation assay using total parasite DNA as probe. We show also that intra-specific DNA variability occurs in T. solium isolates obtained from different geographical areas and discuss the potential significance of this heterogeneity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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