Column and thin-layer chromatographic methods for the simultaneous determination of acediasulfone in the presence of cinchocaine, and cefuroxime in the presence of its hydrolytic degradation products
| Autor: | Mohammad Abdul-Azim, Mohammad, Nagwan H, Zawilla, Fawzy M, El-Anwar, Samir M El-moghazy, Aly |
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| Rok vydání: | 2007 |
| Předmět: |
Dosage Forms
Cefuroxime Dibucaine Reproducibility of Results Reference Standards Sensitivity and Specificity Chemistry Techniques Analytical Drug Stability Models Chemical Ammonium Hydroxide Hydroxides Spectrophotometry Ultraviolet Chromatography Thin Layer Sulfones Chromatography Liquid Densitometry |
| Zdroj: | Journal of AOAC International. 90(2) |
| ISSN: | 1060-3271 |
| Popis: | Column liquid chromatography (LC) and thin-layer chromatography (TLC)-densitometry methods are described for simultaneous determination of acediasulfone (Ace) and cinchocaine (Cinco). In the LC method, the separation and quantitation of the 2 drugs was achieved on a Zorbax C8 column (5 microm, 150 x 4.6 mm id) using a mobile phase composed of methanol-phosphate buffer, pH 2.5 (66 + 34, v/v), at a flow rate of 1 mL/min and ultraviolet detection at 300 and 327 nm for Ace and Cinco, respectively. The method showed linearity over concentration ranges of 20-200 and 45-685 microg/mL, respectively. In the TLC-densitometry method, a mobile phase composed of methanol-tetrahydrofuran-acetic acid (45 + 5 + 0.5, v/v/v) was used for the separation of the 2 drugs. The linearity range was 0.5-4 and 2-9 microg/spot, respectively. In addition, stability indicating TLC-densitometry method has been developed for determination of cefuroxime sodium in the presence of 5-70% of its known hydrolytic degradation products. The mobile phase butanol-methanol-tetrahydrofuran-concentrated ammonium hydroxide (50 + 50 + 50' + 5, v/v/v/v) was used. The concentration range was 2-10 microg/spot. The optimized methods proved to be specific and accurate for the analysis of the cited drugs in laboratory-prepared mixtures and dosage forms. The obtained results agreed statistically with those obtained by the reference methods. |
| Databáze: | OpenAIRE |
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