| Autor: |
X J, Chen, Y, Zhang, G X, Jia, Q G, Meng, T D, Bunch, G S, Liu, S E, Zhu, G B, Xhou |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Cryo letters. 37(2) |
| ISSN: |
0143-2044 |
| Popis: |
Antioxidants protect spermatozoa against cell damage during cryopreservation.To investigate whether melatonin supplement in the extender may improve the quality of cryopreserved mouse sperm.Kunming mice sperm frozen in extender R18S3 (18% (w/v) raffinose and 3% (w/v) skim milk) supplemented with melatonin were thawed and evaluated.Mouse spermatozoa were cryopreserved in the freezing extender R18S3 that contained melatonin at 0, 0.125, 0.25 and 0.5 mg/mL melatonin. The extender without melatonin supplement was associated with increased formation of reactive oxygen species (ROS) and decreased sperm motility. Melatonin supplement at 0.125 mg/mL significantly increased the progressive motility of sperm in comparison to other melatonin concentration or control. The percentage of thawed viable sperm with ROS was lower in the melatonin-treated groups than in untreated group. Melatonin supplement also increased antiapoptotic gene Bcl-xl expression in the thawed sperm.Supplement of 0.125 mg/mL melatonin could reduce oxidative damage and apoptosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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