| Autor: |
S, Sellman, T G, Blanchard, J G, Nedrud, S J, Czinn |
| Rok vydání: |
1995 |
| Předmět: |
|
| Zdroj: |
European journal of gastroenterologyhepatology. 7 |
| ISSN: |
0954-691X |
| Popis: |
To examine the level and duration of the humoral immune response to Helicobacter felis following oral immunization or infection.Germ-free mice were orally immunized with sonicated H. felis plus cholera toxin five times over 6 weeks. One week after immunization was completed, immunized and control non-immunized mice received an oral challenge of live H. felis organisms. The animals were killed at 3-week intervals and serum, gastric washings, intestinal washings and gastric biopsies were obtained. H. felis infection was confirmed by a positive urease test or culture of the gastric biopsy. Serum gastric and intestinal antibody titers were determined by enzyme-linked immunosorbent assay.Infection and immunization against H. felis produces a specific humoral response. The humoral response in infection alone is significantly smaller than that of immunized animals until 6 weeks after infection. The humoral response following oral immunization persists for at least 18 weeks without further stimulation. The presence of an H. felis-specific antibody immune response before infection may be needed to protect animals from acute Helicobacter infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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