Molecular characterization of the rat insulin enhancer-binding complex 3b2. Cloning of a binding factor with putative helicase motifs
| Autor: | S Y, Shieh, C M, Stellrecht, M J, Tsai |
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| Rok vydání: | 1995 |
| Předmět: |
Cell Nucleus
Base Sequence Sequence Homology Amino Acid Molecular Sequence Data DNA Helicases Regulatory Sequences Nucleic Acid Blotting Northern Transfection Recombinant Proteins Cell Line Rats DNA-Binding Proteins Islets of Langerhans Mice Gene Expression Regulation Cricetinae Animals Humans Insulin Amino Acid Sequence Cloning Molecular Gene Library Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 270(37) |
| ISSN: | 0021-9258 |
| Popis: | Cell-specific expression of the rat insulin II gene is in part mediated through an element located in the 5'-flanking region. The element, termed RIPE3b (-126 to -101), confers beta-cell-specific expression in conjunction with an adjacent element RIPE3a (-110 to -86). Here we report the characterization of one of the RIPE3b-binding complexes, 3b2. UV cross-linking analysis demonstrated that it is composed of at least three polypeptides: p58, p62, and p110. Furthermore, a cDNA was isolated via expression screening for binding to RIPE3b. Sequence analysis reveals that the encoded protein, designated Rip-1, possessed putative helicase motifs and a potential transcription activation domain. Overexpression of Rip-1 in cells greatly enhances the 3b2 binding complex, suggesting that Rip-1 is involved in the binding of 3b2. |
| Databáze: | OpenAIRE |
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