Characterization of wild-type and Ser53 mutant eukaryotic initiation factor 4E overexpression in mammalian cells
Autor: | R J, Kaufman, P, Murtha-Riel, D D, Pittman, M V, Davies |
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Rok vydání: | 1993 |
Předmět: |
Base Sequence
Macromolecular Substances Genetic Vectors Molecular Sequence Data Gene Expression Kidney Transfection Recombinant Proteins Cell Line Eukaryotic Initiation Factor-4E Methionine Eukaryotic Initiation Factor-4F Oligodeoxyribonucleotides Peptide Initiation Factors Protein Biosynthesis Chlorocebus aethiops Mutagenesis Site-Directed Serine Animals Electrophoresis Polyacrylamide Gel Amino Acid Sequence Isoelectric Focusing Phosphorylation |
Zdroj: | The Journal of biological chemistry. 268(16) |
ISSN: | 0021-9258 |
Popis: | Eukaryotic translation initiation factor 4E (eIF-4E) is one component of the m7G-cap-binding protein complex eIF-4F and is required for cap-dependent translation initiation. The phosphorylation state of eIF-4E correlates with increased activity and a major phosphorylation site resides at serine 53. To further evaluate the role of eIF-4E phosphorylation, eIF-4E wild-type and two Ser53 mutants, Ser53Ala and Ser53Asp, were expressed at high level, representing almost 2% of the total cell protein, by transient transfection of COS-1 monkey cells. 32PO4 metabolic labeling of transfected cells demonstrated both Ser53 mutants were phosphorylated at an alternate serine residue. [35S]Methionine pulse-labeling demonstrated that the wild-type and both Ser53 mutants were equally incorporated into the eIF-4F complex. The effect of wild-type and Ser53 mutant overexpression on cap-dependent translation initiation and internal translation initiation was monitored by cotransfection with an expression vector encoding a dicistronic mRNA for which the 5' cistron is translated in a cap-dependent manner, and the 3' cistron is translated by internal ribosome binding. Unexpectedly, overexpression of the wild-type or either mutant did not affect the efficiency of either cap-dependent or internal initiation. These results demonstrate that phosphorylation of eIF-4E at residue 53 is not required for interaction with p220 and suggest that Ser53 phosphorylation may not be required for cap-dependent translation initiation in this system. |
Databáze: | OpenAIRE |
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