| Autor: |
Anna, Sledzińska, Lynsey, Fairbairn, Thorsten, Buch |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1193 |
| ISSN: |
1940-6029 |
| Popis: |
Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out modification at specific sites in the DNA of cells. The system consists of an enzyme, Cre recombinase, that recombines a pair of short target sequences, the lox sites. The Cre-Lox system can be used to activate or repress a gene depending on the placement of the lox sites. Placing the Cre recombinase under the control of a cell-specific promoter allows expression only in specific cells or cellular subsets, thus providing a powerful tool for analysis of gene function at specific developmental or physiological niches. Nowadays almost every aspect of T cell biology can be approached by a specific Cre model. This powerful tool allows scientists to overcome the limitations of gene-deficient animals and target a gene of interest specifically in T cell or T cell subsets by appropriate placement of the lox sites. Here we describe the main Cre lines that enable gene targeting in T helper cells or CD4 T cell subsets, and the most common methods of assessing the recombination efficiency. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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