| Autor: |
Jing, Ye, Zhang-yuan, Liao, Hui, Sun, Xiao-fan, You, Xiao-li, Wu, Cun-jiang, Li |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Zhonghua nei ke za zhi. 50(10) |
| ISSN: |
0578-1426 |
| Popis: |
To evaluate three methods of detecting anti-aquaporin 4(AQP4) antibody in neuromyelitis optical(NMO), including indirect immunofluorescence assay organization (IIF), cell immunofluorescence method (CBA) and ELISA.The patients were divided into NMO group (n = 29), multiple sclerosis (MS) group (n = 23), and healthy controls group (n = 50). IIF, CBA and ELISA were used in 3 groups to detect serum anti-AQP4 antibody. The sensitivity and specificity as well as the consistency of positive results were compared.In the aspect of the sensitivity of the three anti-AQP4 antibody to diagnosis NMO, CBA (72.4%)IIF (62.1%)ELISA (51.7%); in the aspect of specificity, CBA (100.0%)ELISA (98.6%)IIF (97.3%). Kappa testing and evaluation method showed that the three detection methods were all in good consistency, particular in CBA and ELISA (P0.01).CBA method showed a highest specificity and sensitivity in all these three anti-AQP4 antibody detection methods. CBA and ELISA are in better consistency of positive results. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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