Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources

Autor:	Jingli, Xie, Li, Zhang, Qin, Ye, Qingwei, Zhou, Li, Xin, Peng, Du, Renbao, Gan
Rok vydání:	2003
Předmět:	Glycerol Quality Control Methanol Plasminogen Carbon Peptide Fragments Pichia Recombinant Proteins Oxygen Species Specificity Ammonia Humans Cloning Molecular Angiostatins
Zdroj:	Biotechnology letters. 25(2)
ISSN:	0141-5492
Popis:	A recombinant strain of Pichia pastoris with a phenotype of MutS was used to produce angiostatin. Due to the low methanol consumption rate of this strain, both methanol and glycerol feedings, that produced oscillation in dissolved O2 concentration, were used during the expression phase to improve cell growth and angiostatin expression. However, enhanced cell growth led to nitrogen limitation that suppressed further production of angiostatin, but addition of ammonia allowed angiostatin concentration to reach 108 mg l(-1) after an expression period of 96 h. The ratio of consumed glycerol to methanol of 1.5:1 (w/w) in the expression phase suggested that methanol played an important role in the metabolism of carbon sources.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7de404a103fcdf1900087ca06ce0b1b1 https://pubmed.ncbi.nlm.nih.gov/12882295 Zobrazit plný text záznamu Full text from SpringerLink