| Autor: |
Yidan, Song, Yihua, Pan, Mengsong, Wu, Wentian, Sun, Liangyu, Luo, Zhihe, Zhao, Jun, Liu |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Stem cell reviews and reports. 17(6) |
| ISSN: |
2629-3277 |
| Popis: |
This study aimed to explore the regulatory mechanism of methyltransferase3 (METTL3) -mediated long non-coding RNA (lncRNA) N6-methyladenosine (mMethylated RNA immunoprecipitation sequencing (MeRIP-seq) and high- throughput sequencing for RNA (RNA-seq) were performed on hASCs. Osteogenic ability was detected by alkaline phosphatase (ALP) staining, Alizarin Red S(ARS) staining, ALP quantification and Quantitative real-time polymerase chain reaction analysis (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis predicted the osteogenesis-related pathways enriched for the lncRNAs and identified the target lncRNAs. After overexpression and knockdown of METTL3, methylated RNA immunoprecipitation-qPCR (MeRIP-qPCR) and qRT-PCR were used to detect the levels of mIn vitro experiments showed that METTL3 can promote osteogenic differentiation and that its expression level is upregulated. KEGG pathway analysis predicted that lncRNAs with differentially upregulated methylated peaks were enriched mostly in the mitogen-activated protein kinase (MAPK) signaling pathway, in which Serine/threonine protein kinase 3 (STK3) was the predicted target gene of the lncRNA RP11-44 N12.5. The mThis study shows, for the first time, that METTL3 can activate the MAPK signaling pathway by regulating the m |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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