| Autor: |
Lei, Qin, Heng-An, Wang, Zhong-Qin, Wu, Xiao-Feng, Zhang, Mei-Lei, Jin, Zi-Xin, Deng, Guo-Ping, Zhao |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Acta biochimica et biophysica Sinica. 36(8) |
| ISSN: |
1672-9145 |
| Popis: |
The hmr19 gene was cloned from Streptomyces hygroscopicus subsp. yingchengensis strain 10-22, a bacterium strain producing agricultural antibiotics. Sequence similarity comparison indicates that hmr19 gene may encode a predicted protein with 14 putative transmembrane alpha-helical spanners, belonging to the drug:H(+) antiporter-2 family of the major facilitator superfamily. The expression of hmr19 in the mycelium of strain 10-22 was detected by Western blotting analysis. Gene replacement technology was employed to construct an hmr19 disruption mutant. The growth inhibition test against different antibiotics indicated that the mutant strain was 5-20 fold more susceptible to tetracycline, vancomycin and mitomycin C than the parental wild type strain. The mutant took up tetracycline much faster and accumulated more antibiotics than the wild type strain 10-22. While with the addition of an energy uncoupler, carbonyl cyanide m-chlorophenylhydrazone, the characteristics of the accumulation of [(3)H]tetracycline in these two strains were almost the same. It was thus concluded that hmr19 encoded a multidrug resistance efflux protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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